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The next day, wells were washed twice with PBS and blocked with 360 L of 4% (w/v) skimmed milk in PBST (PBS + 0.05% Tween-20) for 1.5 h at room temperature (RT)

Based on a minimum clinically important change (MCIC) of just over 3-point improvement in UPDRS [23], the current model shows an approximate duration of effect for approved doses of SC apomorphine of 60 to 90 min, and just under 90 min for the approved 30-mg dose of SL apomorphine (Fig