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For experiments, both flanks of 3-4 weeks male and female mice Braf V600E/+ /Pten fl/fl ( BPT control), Epac1 fl/fl , Epac2 fl/fl , Epac1 +/fl /Epac2 +/fl , and Epac1 fl/fl /Epac2 fl/fl were shaved and 2 L of 5 mM 4HT was applied topically on both flanks for 2 days to generate BPT, Epac1 and Epac2 or Epac1/2 conditional knockout mice referred to hereafter as BPT, Epac1 +/ /Epac2 +/ , Epac1 / /Epac2 / , Epac1 / , and Epac2 / , respectively

In controlled studies using aging C57BL/6J mice, supplementation beginning at 65 weeks increased lifespan by 23.7% (104.0 vs

[DOI] [PubMed] [Google Scholar] 75.Tosi G.M., Giustarini D., Franci L., Minetti A., Imperatore F., Caldi E., Fiorenzani P., Aloisi A.M., Sparatore A., Rossi R., et al

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