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MDA-MB-453, 100000 cells/mL and BT-474, 35000 cells/mL in a 96-well plate and allowed to adhere for 24 h (HeLa cells), 48 h (MDA-MB-231 cells and MDA-MB-453 cells) or 72 h (BT-474 cells)

Research emphasizes the importance of the endocannabinoid system (ECS) in neurotrophic factor signaling during fetal CNS development, regulating synaptogenesis, axon growth, neuronal cell fate, migration, and proliferation, as well as neuroprotection mediated by CB1R and BDNF, preventing neuronal death, and the ECSs influence on dopaminergic systems (primarily through D2 receptors), GABAergic, and glutamatergic systems (163)

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Fras, JP., Davies, MJ., Rosenstock, J., Prez Manghi, FC., Fernndez Land, L., Bergman, BK., Liu, B., Cui, X., Brown, K.(2021)