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For real-time calcium imaging assay, plates were incubated at 4 C for 2 days in the dark and then transferred to 22 C with a SD photoperiod for 67 days

This significantly revised edition contains 74 chapters covering the full spectrum of metabolic and bariatric surgery, from the disease of obesity and surgical physiology to operative techniques, outcomes, complications, revisional surgery, endoscopic therapies, quality improvement, and the growing role of obesity medications before and after surgery

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