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In most cases, where the fusion protein is initially observed primarily in inclusion bodies, it is desirable to modify culture conditionssuch as lowering growth temperature, increasing aeration, or altering induction conditions toattempt to obtain soluble protein, rather than to solubilize inclusion bodies with denaturants and refold the protein after extraction

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Call a healthcare provider right away if you have any of the following symptoms, especially if they are new, worse, or worry you: 4