To learn more about how column chromatography works, read GoldBio article below: How Column Chromatography Works to Separate Proteins To perform column chromatography, in addition to your sample, you will need a column, a matrix, a solvent to dissolve your sample, a washing buffer, and an elution buffer
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doi:10.1016/j.jconrel.2011.01.030
Kempuraj D, Thangavel R, Natteru P, Selvakumar G, Saeed D, Zahoor H, et al