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FIGURE 7 Effect of GPX3 on stress-induced senescence model in rat tenocytes The role of GPX3 in cellular senescence was also investigated using the stress-induced senescence model created via treatment with H 2 O 2 (Figure 7G)

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1) If the culture supernatant does not contain SDS, urea, or any other denaturing agents that might affect the binding of GST tagged protein and GSH column, it can be applied directly to the column