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For individuals significantly above 250 pounds, extended protocols of 16-20 weeks rather than higher daily doses often produce better outcomes

GHK-CU research concentrations vary by application, with tissue culture studies employing concentrations around 0.3 mg/mL in specific experimental contexts

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Nucleofected cells were recovered with 500 l of complete medium containing 10% FBS and dispensed into a 12 well plate containing 1 ml of pre-warmed media and incubated at 37 C, 5% CO2 until the cells were 80-90% confluent