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Low LH, low estradiol, and low-normal T3 in the presence of normal TSH suggest functional hypothalamic suppression secondary to energy deficit

To test whether spike-induced loss of apical CFTR was due to failure of recovery from endosomal recycling, we used an impermeant biotin labelling method to ask how much CFTR from the apical surface was returned to the apical surface after exposure to progressively increasing concentrations of spike protein

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Without this step, you are pulling liquid out against a vacuum, which makes accurate volume measurement difficult and introduces more air bubbles into the syringe