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BDNF-Adjacent Pathways: While GHK-Cu does not directly upregulate BDNF (Brain-Derived Neurotrophic Factor), its anti-inflammatory effects in the CNS specifically its reduction of microglial activation may create conditions conducive to BDNF signaling

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Description S-Acetyl Glutathione (S-A-GSH) is a unique form of glutathione, one of the most powerful antioxidants naturally produced in the body

Samples were then washed with PBS as described previously and incubated with goat anti-rabbit secondary antibody (1:500 dilution, Beyotime) at room temperature for 1 h in darkness