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Rett syndrome, caused by MECP2 gene mutations, disrupts GABAergic interneuron development, lowering GABA concentrations in the hippocampus and prefrontal cortex, leading to developmental regression, such as loss of language and motor skills, and stereotypic hand movements like hand-wringing (77)

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The instant a needle penetrates the rubber stopper, three things happen: air enters the vial headspace, the sterile barrier is permanently breached, and the multi-dose use clock starts