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Promega) (for AMYRs) or coelenterazine h (Nanolight Technologies, Pinetop, AZ) (for CTR) was then added to cells at a final concentration of 1:1000 or 5 M, respectively, and incubated for a further 1015 min

Most vitamin C and glutathione formulations are best applied to clean skin in the morning, followed by moisturizer and always finished with a broad-spectrum sunscreen, since antioxidants work best as part of a layered defense rather than a replacement for sun protection