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The rhythmicity of melatonin secretion is not very pronounced in young babies, but it becomes more rhythmic over time, and factors such as artificial lighting and lack of sunlight exposure can disrupt this rhythmicity, making people somewhat arrhythmic or phase-shifted 2m6s

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Some researchers also track NAD+ metabolite levels via urinary nicotinamide excretion to confirm functional NNMT inhibition, though this is not standard practice