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The separation was achieved using an RP C8 column, with heptafluorobutyric acid as an ion-pairing reagent to optimize retention, particularly since these compounds are cations under acidic conditions

The first step is to use a high-quality, precise measuring tool, such as an insulin syringe

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Advances in omics technologies have enabled researchers to investigate the components and mechanisms of traditional Chinese medicine (TCM) with greater depth and systematic precision