Organic solvents such as methanol (MeOH), ethanol (EtOH), acetonitrile (ACN), acetone, hexane, chloroform (CHCl 3 ), 2-propanol (iPrOH), acetic acid (AcOH), xylene, toluene, and tert -butyl methyl ether can remove small molecules, thereby improving in situ protein detection and imaging by reducing ion suppression from salts, lipids, and metabolites
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This effect typically diminishes within 4-8 weeks