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Freezing should be avoided at all stages because it may damage container integrity and affect product quality

10.1152/physrev.00022.2020 54 CoelhoF

This NIH-funded study stands as a documented case of profound ethical and regulatory failure and demands immediate, independent federal review, full transparency, and corrective action to protect public health and restore trust in human subjects research

A 2017 study examined cultured vascular endothelial cells, a chick chorioallantoic membrane model, and a standardized rat hind-limb ischemia model