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After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

Zhang, L., Tan, J., Han, D

It is now possible to determine the concentration of glutathione (GSH) in living human brains using magnetic resonance spectroscopy (MRS), with multiple MRS studies showing depleted brain glutathione (GSH) levels in all the major neurodegenerative diseases [6-9]

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