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Their pleiotropic effects beyond glycemic control offer promising therapeutic opportunities in chronic skin diseases and plastic surgery, but also introduce unique challenges related to cutaneous adverse reactions, facial aging, surgical outcomes, and perioperative safety

For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

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