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Further, methods like thermal analysis techniques and DSC help in studying thermal stability, and other thermal transitions in the material to determine the thermal stability in case of changes in temperatures

Membranes were blocked in 5% (w/v) milk in PBS-T (PBS with 0.1% [v/v] Tween-20) for 1 h, incubated overnight at 4 C with the primary antibody in the same blocking solution, washed three times with PBS-T for 5 min, incubated with HRP- or Alexa Fluor (AF)-conjugated secondary antibody (where indicated) in 0.1% (w/v) milk in PBS-T for 1 h and, washed three times with PBS-T for 5 min