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Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco), 1 mM sodium pyruvate (Gibco) and 100 units/mL penicillin and 100 g/mL streptomycin at 37 C in 5% CO 2

Once stabilized, cats with hypertension should be re-evaluated at least every three to four months, or as dictated by any concurrent diseases

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