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Tambini says

Use top-down sequencing when modification mapping and intact structure are critical Use bottom-up sequencing for routine, high-throughput, and easier analysis In many cases, a hybrid approach combining both methods provides the most comprehensive results 5: Key Challenges in Peptide Sequencing of GLP-1 Peptide The key challenges in Peptide Sequencing of GLP-1 Peptide primarily arise from complex fragmentation behavior and the difficulty of accurately detecting structural modifications and low-level impurities

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