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In cases of significant level disturbances, nausea, vomiting, drowsiness or confusion have been reported, together with abnormal test results (for example, elevated transaminases)

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Protein analysis of biopsy samples Muscle biopsy samples were allocated for protein determination by western blot analysis with antibodies (New England BioLabs, Mississauga, ON, Canada) against total and the phosphorylated protein targets: mTOR, phospho-mTOR (Ser2448), p70-S6K, phospho-p70-S6K (Thr389), 4E-BP1, and phospho-4E-BP1- (Thr37/46)

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