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Here we report on the development and characterization of small-molecule inhibitors of GSTO1 and the use of these inhibitors to study the cellular functions of GSTO1

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Immunopurified proteins were mixed with methylation reaction buffer (50 mM Tris-HCl, pH 8.0, 20 mM KCl, 5 mM DTT, 4 mM EDTA) in the presence of 200 M S-adenosyl-L-methionine (SAM, Sigma) at 37 C for 1 h