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Western blotting The hippocampus tissue extracts were prepared using Protein extraction reagent (Thermo Fisher Scientific, 78501) and centrifuged at 12,000 rpm for 10 min at 4 C

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If subcutaneous administration isn't feasible for the research model, consider encapsulation technologies or absorption enhancers for MOTS-c, though published data on oral MOTS-c efficacy remains limited compared to injectable protocols