To visually observe the live and dead cells induced by different nanocomposites, we stained the cells with Calcein AM (acetoxymethyl ester of calcein, green fluorescence indicates live cells) and propidium iodide (PI, red fluorescence indicates dead cells) [36]
Data were acquired using an MS3-based TMT method using the following scan parameters: scan sequence began with an MS1 master scan (Orbitrap analysis, resolution 120,000, 4001700 m/z, RF lens 60%, automatic gain control [AGC] target 2E5, maximum injection time 50 ms, centroid mode) with dynamic exclusion enabled (repeat count 1, duration 15 s)
A specialist librarian was recruited to validate the search string derivation
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