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Calibration standards were prepared by spiking 50 L of human plasma with appropriate amount of thiols to obtain the following concentrations: 20, 40, 60, 100, 200, 300, 600 nmol/mL plasma for Cys, 2, 4, 6, 10, 20, 30, 60 nmol/mL for Hcy, GSH and 0.1, 0.5, 1.0, 2.0, 4.0, 5.0, 10.0 nmol/mL for N-Hcy-Lys

This was achieved using experimental [GSH] and [GSSG] data consisting of measurements at different postnatal days from PN11 up to PN28 from the retinas of control C3H mice and the rd1 mouse model for the disease RP

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If you decide to take a B12 multivitamin complex, try getting the pure forms without any added junk in capsules or injection solutions