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Real-time polymerase chain reaction was performed with the Applied Biosystems 7900HT Fast Real-Time PCR system, using Fast SyBr Green Master Mix (Applied Biosystems) and specific human primers to GAPDH, IL-6, MCP-1, TNF, VCAM-1, PAI-1, thrombin, angiopoietin-2, TGF, preproET-1, SOD-1, catalase, glutathione peroxidase, peroxiredoxin, heme oxygenase-1 and thioredoxin

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Using engineered immune cells, such as macrophages or T cells, as delivery vehicles for tumor-homing siRNAs could significantly improve target specificity

The regulatory status of BPC-157 in Canada mirrors other Western nations