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Both uniformly C13-labeled palmitic acid (UC13-C16:0, Larodan) and docosahexaenoic acid (DHA, Cayman Chemicals) were conjugated with albumin (using Fatty Acid Free Bovine Serum Albumin, BSA, Goldbio) to a final concentration of 2 mM and 40 M, respectively, and aliquoted out into 5 ml glass scintillation vials stored at 20C

Cells were washed three times in ice cold FACS buffer, fixed in 4% paraformaldehyde (PFA) for 20 min and washed a further two times in FACS buffer

Medium-Term Storage (up to one month): Aliquot and store at -20C

For administering the final dose, a U-100 insulin syringe is the standard due to its fine gradations for accurate dosing