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Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

Computational drug repositioning approaches may be employed for the development of new treatment strategies for Alzheimers patients since an extensive amount of omics data has been generated during pre-clinical and clinical studies

Detector type set to Orbitrap, with 60 K resolution, with wide quad isolation, mass range = normal, scan range = 3001500 m/z, max injection time = 50 ms, AGC target = 200,000, microscans = 1, S-lens RF level = 60, without source fragmentation, and datatype = positive and centroid