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SPP1 exacerbates ARDS via elevating Th17/Treg and M1/M2 ratios through suppression of ubiquitination-dependent HIF-1alpha degradation

In the current study, blots were incubated with IGF-1R (1:1000), cone arrestin (1:1000), transducin (1:1000), rhodopsin (1:10,000), rod arrestin (1:1000), M-opsin (1:1000), and actin (1:1000) antibodies (see Table S3) overnight at 4 C