Generally, following the purification of phenolics, the samples are analyzed using a C18 column as the stationary phase ( Several factors affect the sensitivity or effectiveness of HPLC, including phenolic purification steps, mobile phase composition, column selection, and pre-concentration procedures ( HPLC run times typically range from 10 to 150 min
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Mohamed MM, Sabet S, Peng DF, Nouh MA, El-Shinawi M, El-Rifai W