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Strengths and limitations The strengths of our study are as follows: (1) We performed a robust, streamlined proteomics method to quantify proteins in the human serum using nano UHPLC-MS/MS 15

We used GHRP-6, a ghrelin receptor agonist, as a ghrelin mimetic, because in this experiment we needed continuous administration of ghrelin for more than 48 h, and GHRP-6 is more stable than ghrelin