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(This medication was previously called Accutane, but this brand-name version is no longer on the market.) Isotretinoin can be a miracle cure for acne, but it comes with some major side effects and health risks, such as increased sun sensitivity, eye damage, blood sugar spikes, behavior changes, anxiety, muscle problems, or the dreaded Accutane purging

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Cells were re-suspended in flow cytometry buffer (PBS, 0.5% BSA, 0.02% NaN3, 1 mM EDTA) and cell death and cell cycle phases were analyzed by flow cytometry in a BD FACSVerseTM flow cytometer (Becton Dickinson AG, Allschwil, Switzerland)

3b was obtained after purification by column chromatography in a yield of mg 61.3 mg (0.4 mmol, 81.1%)