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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

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Plasma TMAO, and total carnitine (TC) levels were measured by the liquid chromatography-mass spectrometry system at the Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences (Warsaw, Poland) as described previously [18, 19]

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