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When compared with placebo or other therapies, GLP-1 agonists were associated with an increased risk of biliary disease [23]: Overall biliary disease: RR 1.37(95% CI 1.231.52) Cholelithiasis: RR 1.27 (95% CI 1.101.47) Cholecystitis: RR 1.36 (95% CI 1.141.62) Mechanisms: Reduced gallbladder motility Rapid weight loss increased cholesterol saturation and gallstone formation

The daily versus weekly application creates an adherence difference that can explain part of the effect differences in real-world data

Statistical analysis was performed by t -test (unpaired or paired, as indicated), one-way analysis of variance (ANOVA), two-way ANOVA (time treatment or genotype treatment) with post hoc comparisons (where appropriate, as indicated in the figure legends)

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