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doi: 10.1007/s11096-015-0134-z 43

Samples in extraction mix were vortexed for 10 min at 4 C and centrifuged at 15,000 rpm for 10 min at 4 C to pellet insoluble material

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Interestingly however, the receptor was not completely unphosphorylated under vehicle conditions, suggesting that there is some basal level of GRK recruitment and phosphorylation present under unstimulated conditions, with the receptor potentially cycling between phosphorylated and unphosphorylated states by a currently unknown mechanism