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Second, as a single-center study with limited omics sample size, future validation in larger cohorts is warranted to confirm ITIH3 and ACAT1 as biomarkers

2.5 Assessment of Activity of Antioxidant Enzymes in Testicular Tissues The tissues were thawed and homogenized in 10% w/v ice-cold 0.05 m potassium phosphate buffer (pH 7.4)

Vitamin K3 (menadione) has demonstrated the ability to suppress epithelialmesenchymal transition and the Wnt signaling pathway in colorectal cancer cells, decreasing cell proliferation, invasion, and migration (101)