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The homogenate was centrifuged at 4000 g for 15 min and assayed immediately or frozen at 80 C in small aliquots and used after thawing within no more than a month

This condition often coexists with other mental and sleep disorders, such as restless legs syndrome (RLS) [70]

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Schnemann HJ, Vist GE, Higgins JP, Santesso N, Deeks JJ, Glasziou P, et al