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When methionine intake suddenly increases (whether dietary or via injection), the transsulfuration pathway that converts homocysteine to cysteine and eventually glutathione becomes rate-limited by the availability of vitamin B6 (pyridoxal-5-phosphate) and folate (5-MTHF)

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According to the NIH, common doses range from 3 milligrams (mg) to 5,000 mg daily, with an average 14% to 18% uptake rate