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doi: 10.3389/fphar.2024.1489877 Received 02 September 2024 Accepted 21 October 2024 Published 30 October 2024 Volume 15 - 2024 Edited by Ana Raquel Santiago, University of Coimbra, Portugal Reviewed by Aarti Sharma, Mayo Clinic Arizona, United States Pere Garriga, Universitat Politecnica de Catalunya, Spain Updates Copyright 2024 Hao, Xu, Zhang and Xue

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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

This is why mixing whey protein and creatine in the same shake is a convenient and effective supplementation strategy