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However, compared to ChlaDUB1 Ub binding, in SnCE1 specificity towards SUMO1 is created by formation of several side-chain interactions of SUMO1 with SnCE1 such as side chain of Gln94 #2 with the hydroxyl group of side chain Thr146 1 in VR-2, Gln29 #2 with main chain of Thr146 1 , side chain of Tyr91 #2 with main chain of Leu308 #1 in the SnCE1 C-terminal region and side chain of Glu89 #2 with the imidazole side chain of His163 #1 (Fig

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Detection methods include mass-spectrometry-based urine and blood assays that can identify exogenous IGF-1 analogues by their structural differences from endogenous hormone [21]