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That's why you see more visible change after every session when we add it to your protocol."

4.5 mg arm: 10.8% mean loss

This is in accordance with our Cu ion content measurement of the PBS and bacterial solution after wet contact killing on Cu-Al alloy samples (Supplementary Figure 4), which is most likely contributing to the lower antibacterial effect, as described in see section 4.4 Bacterial survival after long exposure times is higher within a bacterial community. Another alloy of interest would be Al-Ag-Cu, which has shown to have a greater antibacterial effect compared to Cu-Al alloys on strains of E

& Majcherczyk, A