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Amplification mixes were added into each dish and incubated at 37 C for 100 min in the dark

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Once breakdown starts, a protein called intrinsic factor, released by parietal cells in the stomach, binds to vitamin B12 in the duodenum (small intestine) and forms a B12-intrinsic factor complex that allows B12 to be absorbed in the terminal portion of the small intestine

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