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If you reconstituted more than you will use within one to two weeks, consider dividing the solution into single-use aliquots before freezing, as covered in depth in peptide refrigeration duration guides

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Western blot Cells were lysed in 1 RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min

Oxidation of fatty acids Oxidation of fatty acids is controlled by PPAR and occurs mainly in the mitochondria, providing a source of energy to generate ATP especially when circulating glucose concentrations are low [14, 77,78,79,80,81]