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An internal standard (IS) solution, containing 13C isotope-labelled GSH and 13C isotope-labelled GSSG, was prepared in an antioxidation buffer

10.1007/s00232-011-9382-6 96 NesicO.LeeJ.UnabiaG

These doses were determined based on studies that measured the amounts of niacin metabolites excreted in the urine (e.g., N1-methylnicotinamide)

Considering that the water molecules were trapped largely in the hydrophobic pockets, often made contacts with or were displaced by the fragments, and did not appear to play a structural role, we anticipated that their displacement with larger non-polar moieties would improve binding of newly designed inhibitors