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More importantly, using human embryonic stem cell (hESC) culture models, researchers showed that these mitochondrial dysfunction signatures occur in HD-affected hESCs but not HD-affected hESCs-derived neural lineage cells, while a shift to transcriptional dysregulation and cytoskeletal abnormalities, the primary HD pathologies, appears in HD-affected differentiating hESCs along neural lineages in vitro (Monk and Connor, 2021)

As soon as the supernatant was centrifuged, 400 mL of the supernatant was poured into the corresponding EP tube and stored overnight at 20 C in the refrigerator before being centrifuged at 12,000 r/min for 3 min at 4 C, and approximately 2000 mL of supernatant was collected in the injection bottle liner for on-board analysis

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