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H 2 O 2 produced by oxidative stress can bind to the cysteine 81 site of ATG4 to regulate the activity of ATG4 (Scherz-Shouval et al., 2007), then enhance autophagy and cause autophagic cell death (Koike et al., 2008)

What makes this interesting is that these improvements weren't just about the numbers on the scale - the dihydroberberine plus exercise group showed superior results in both fat loss and muscle preservation compared to exercise alone, pointing to more favorable overall body composition changes

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