Figure 1 The biological effects of testosterone are mediated both by direct binding and activation of the androgen receptor, as well as indirectly through its metabolic conversion to biologically active derivatives, including 5-dihydrotestosterone (DHT) via 5-reductase and oestradiol via aromatization, which subsequently act through androgen and oestrogen receptors (ER/ER), respectively
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The positive electrospray ionization mode was utilized with the following parameters: 325 C for drying gas temperature, 7 L/min for drying gas flow, 45 psi for nebulizer pressure, 325 C for sheath gas temperature, 11 L/min for sheath gas flow rate, and 3500 V for capillary voltage
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