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Science 303 : 16811683

41,42 Although humans can excrete small amounts of mercury in urine or feces as well as via exhalation or sweating, they lack an active robust mechanism for mercury excretion, allowing levels to accumulate with chronic exposure

Intein Clustering Suggests Functional Importance in Different Domains of Life

Lentivirus preparation and transduction To prepare lentivirus particles, 4 10 5 HEK293T cells were seeded into six-well plates and transfected the next day with 0.66 g expression plasmid, 0.44 g pCMV-dR8.91 (gag/pol) and 0.88 g pMD.G vesicular stomatitis virus G glycoprotein using 8 l FuGENE HD transfection reagent (Promega)