Acetylation occurs in multiple functional domains of NRF2, particularly within the transactivation domain and other critical structural regions [54]
Its not just presence or absence of the substance use in and of itself

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Doseresponse effect of interleukin (IL)-1, tumour necrosis factor (TNF)-, and interferon- on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells